chicken anti bdnf  (R&D Systems)

Journal: Cell reports

Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss

doi: 10.1016/j.celrep.2018.09.077

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Chicken polyclonal anti-BDNF , Promega , Cat#G1641; RRID:AB_430850.

Techniques: Virus, Recombinant, Transfection, Western Blot, Sequencing, Plasmid Preparation, Expressing, Software

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: Plasma BDNF levels according to sex and diagnostic group. BDNF levels in plasma (50 ul) were determined by ELISA. (A) BDNF levels did not differ by sex among subjects included in our study as per Student’s t test, unpaired; two-tailed. (B) No significant differences were detected according to diagnostic group between premanifest (PM), manifest HD (HD), and normal controls (NC) as per One-way ANOVA. Data is shown as individual BDNF levels ± SD.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: Clinical Proteomics, Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: Correlations between plasma BDNF levels and clinical data in HD patients and control subjects.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: Clinical Proteomics, Control

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: Salivary BDNF levels according to sex and diagnostic group. BDNF levels in saliva (50 ul) were determined by ELISA. (A) No significant differences in BDNF were observed by sex (Student’s t test, unpaired; two-tailed). (B) BDNF levels are lower in premanifest (PM) and manifest HD (HD) cases when compared to normal controls (NC). ∗ p < 0.05; ∗∗ p < 0.01 in comparison to NC group as per One-way ANOVA. (C) BDNF levels are significantly lower in subjects who are >10 years from their predicted age of onset compared to those who are <10 years from predicted onset. ∗ p = 0.05. Predicted age to onset was calculated using the Langbehn formula . Data is shown as individual BDNF levels ± SD.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Comparison

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: Correlations between salivary BDNF levels and clinical data.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: Methylation sites in the BDNF promoter IV region. (A) Schematic representation of human BDNF gene indicating alternative promoters. CpG sites analyzed are detailed in enlarged box. Position is in relation to transcription start site. (B) Detailed sequence of BDNF promoter IV. Underlined text in yellow boxes indicate regions analyzed with each probe by pyrosequencing. Genomic position is based on chromone build GRCh37.p13 primary assembly.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: Methylation, Sequencing

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: DNA methylation status at selected CpG sites from BDNF promoter IV.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: DNA Methylation Assay

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: DNA methylation levels of BDNF promoter IV in blood. Methylation was quantified by pyrosequencing and expressed as mean percent methylation representing the averaged value of cases (HD Gene+) or control subjects (NC) for each of individual CpG. Data represents mean value ± SEM. ∗ p < 0.05 as per two-tailed Student’s t -test.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: DNA Methylation Assay, Methylation, Control, Two Tailed Test

Journal: Frontiers in Molecular Neuroscience

Article Title: Evaluation of Biochemical and Epigenetic Measures of Peripheral Brain-Derived Neurotrophic Factor (BDNF) as a Biomarker in Huntington’s Disease Patients

doi: 10.3389/fnmol.2019.00335

Figure Lengend Snippet: Correlations between BDNF promoter IV methylation and clinical measures in HD patients.

Article Snippet: Subsequently, a polyclonal chicken anti-human BDNF (100 ul; 2.5 μg/ml; Promega) was added to the plate and incubated for 2.5 h. The plate was washed five times with PBST, then anti-chicken IgY-HRP (1 μg/ml; Promega) was added to each well for a 1-h incubation at RT.

Techniques: Methylation